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In this study we have used cells expressing LFA-3 or T11TS, the human and sheep forms of the ligand of CD2, as well as the purified LFA-3 and T11TS molecules themselves to study their effects on T cell activation via the CD2-mediated "alternative pathway". Sheep red blood cells, which bind to CD2 via T11TS in E-rosette formation, and human autologous monocytes, which express the LFA-3 molecule, both induce proliferation of resting T cells in the presence of per se submitogenic concentrations of anti-T11(2) plus anti-T11(3) monoclonal antibodies (mAb). This effect is blocked by mAb to LFA-3, T11TS and CD2 known to inhibit CD2-ligand interaction. In addition, purified LFA-3 and T11TS, when added at ng amounts to cultures containing submitogenic concentrations of anti-T11(2 + 3) mAb, are also strongly mitogenic for resting human T cells. Thus, both LFA-3 and T11TS are potent co-stimulators of the alternative pathway of T cell activation but by themselves do not provide a mitogenic signal. This finding is discussed with regard to a physiological role of CD2-LFA-3 interaction in T cell activation.

Original publication

DOI

10.1002/eji.1830171227

Type

Journal article

Journal

European journal of immunology

Publication Date

12/1987

Volume

17

Pages

1847 - 1850

Addresses

Genzentrum der Ludwig Maximilians Universität München, Martinsried, FRG.

Keywords

T-Lymphocytes, Monocytes, Animals, Sheep, Carrier Proteins, Lymphocyte Function-Associated Antigen-1, Receptors, Immunologic, Antigens, CD2, Antigens, Differentiation, T-Lymphocyte, Antigens, Surface, Ligands, Lymphocyte Activation, Cell Adhesion, Antigen-Antibody Reactions