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One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell-based assays to experimentally assess the role of dynamin-2 during these processes. Overall, our data suggest that dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion.

Original publication

DOI

10.1016/j.celrep.2016.12.032

Type

Journal article

Journal

Cell Rep

Publication Date

10/01/2017

Volume

18

Pages

443 - 453

Keywords

Dynamin-2, HIV-1 fusion, advanced light imaging, cell-cell fusion, number and brightness