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MicroRNAs (miRNAs) have emerged as key players in the degradation of target mRNAs. They have been associated with diverse biological processes, and recent studies have demonstrated that miRNAs play a role in inflammatory responses. The identification of miRNA and their corresponding messenger RNA (mRNA) targets can therefore be very helpful. In this chapter, we first overview the field of miRNAs and then show the fundamental techniques for the identification of miRNAs and confirmation of their role on target mRNAs.

Original publication

DOI

10.1007/978-1-4939-0404-4_7

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2014

Volume

1142

Pages

55 - 63

Addresses

University of Strasbourg, 1 Avenue Molière, 67000, Strasbourg, France.

Keywords

Animals, Humans, Arthritis, Rheumatoid, MicroRNAs, RNA, Messenger, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Cell Culture Techniques, Transfection, Gene Expression Regulation, Real-Time Polymerase Chain Reaction