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The effect of bradykinin (BK) on proteinase activity, prostaglandin synthesis, and the production of interleukin-6 (IL-6) was investigated in cultures of human osteoblast-like cells. Bradykinin had no effect on stromelysin activity and plasminogen activator activity produced by human osteoblast-like cells. However, BK stimulated the production of prostaglandin E2, an effect that was markedly enhanced by pre-incubation with recombinant interleukin-1 alpha (rhIL-1 alpha), but was apparently unaffected by BK receptor antagonists types 1 and 2. Bradykinin stimulated the intracellular accumulation of total inositol phosphates suggesting that its effects were mediated by stimulation of phosphoinositide metabolism. Bradykinin within the dose range of 10(-11)-10(-5) M also significantly stimulated the production of IL-6. Bradykinin may, therefore, mediate a variety of responses in bone under both physiological and pathological conditions.

Original publication




Journal article


Biochim biophys acta

Publication Date





97 - 102


Bradykinin, Cells, Cultured, Dinoprostone, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Humans, Inositol Phosphates, Interleukin-6, Kinetics, Matrix Metalloproteinase 3, Metalloendopeptidases, Osteoblasts, Signal Transduction