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The stimulation of osteocalcin synthesis by human osteoblast-like cells in response to 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is antagonised by several bone regulatory agents. We have shown that agents which activate adenylate cyclase inhibit this action of 1,25(OH)2D3 on human osteoblast-like cells. Activation of adenylate cyclase, either via the stimulatory GTP-binding protein using cholera toxin, or directly at the catalytic via the stimulatory GTP-binding protein using cholera toxin, or directly at the catalytic subunit using forskolin, results in a suppression of osteocalcin synthesis. Whilst the activation of adenylate cyclase induces this inhibitory response, neither exogenous dibutyryl cyclic AMP nor the phosphodiesterase inhibitor, IBMX, exerted any apparent effect on the production of osteocalcin. The tumour promoting phorbol ester, 4 beta-phorbol 12,13-dibutyrate, also inhibited 1,25(OH)2D3-stimulated osteocalcin production. This was not apparent in response to the non-tumour promoting phorbol ester 4 beta-phorbol suggesting the involvement of protein kinase C.

Original publication

DOI

10.1016/0006-291x(89)91779-8

Type

Journal article

Journal

Biochem biophys res commun

Publication Date

15/11/1989

Volume

164

Pages

1076 - 1085

Keywords

1-Methyl-3-isobutylxanthine, Adenylyl Cyclases, Alkaline Phosphatase, Bone and Bones, Bucladesine, Calcitriol, Cells, Cultured, Colforsin, Humans, Interleukin-1, Kinetics, Osteoblasts, Osteocalcin, Phorbols, Recombinant Proteins, Theophylline