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Uptake of orthophosphate (Pi) by osteoblast-like cells is known to be stimulated by parathyroid hormone (PTH), but effects on intracellular [Pi] have not been investigated. Here we show in rat osteoblast-like cells (UMR 106-06) that PTH (10(-11) to 10(-7) M) increases both 32Pi uptake and cellular [Pi] by up to 50 per cent. 1,25 Dihydroxyvitamin D3 (1,25D) (10(-12) to 10(-6) M) and salmon calcitonin (CT) (10(-12) to 10(-6) g ml-1) also increased cellular [Pi] (by up to 60 per cent), but the percentage increases in total cellular 32Pi uptake were smaller. The effects of 1,25D were transient (observable at 80 min and 6 h but not 24 h), and were also observed with 24,25 dihydroxy- and 25 hydroxyvitamin D3. Transient degradation of organic phosphorus pools to Pi might contribute to this increased [Pi]. These pools remain to be identified but were not shown to be phospholipids. Foetal bovine serum also affected cellular [Pi]. Care is therefore needed in distinguishing direct hormonal effects on cellular [Pi] from indirect effects arising from changes in the rate of cell growth.

Type

Journal article

Journal

Cell biochemistry and function

Publication Date

03/1993

Volume

11

Pages

25 - 34

Addresses

Department of Human Metabolism and Clinical Biochemistry, Medical School, Sheffield, U.K.

Keywords

Tumor Cells, Cultured, Osteoblasts, Animals, Cattle, Rats, Phosphates, Calcium, Calcifediol, Calcitriol, 24,25-Dihydroxyvitamin D 3, Calcitonin, Parathyroid Hormone, Membrane Lipids, Phospholipids, Second Messenger Systems, Biological Transport, Blood Physiological Phenomena