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We report a novel, compact and automated multidimensional spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Förster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

Original publication

DOI

10.1002/jbio.200810051

Type

Journal article

Journal

Journal of biophotonics

Publication Date

12/2008

Volume

1

Pages

494 - 505

Addresses

Chemical Biology Centre, Department of Chemistry, Imperial College London, London, UK. hugh.manning05@imperial.ac.uk

Keywords

Cartilage, Cell Membrane, Humans, Pyridinium Compounds, Lipid Bilayers, Fluorescent Dyes, Microscopy, Fluorescence, Multiphoton, Spectrometry, Fluorescence, Fluorescence Resonance Energy Transfer, Equipment Design, Protein Interaction Mapping, Optical Fibers, Photochemical Processes, In Vitro Techniques