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The three-dimensional organization of the ubiquitous extracellular matrix glycoprotein fibronectin regulates cell fate and morphogenesis during development; in particular tubule formation that constitutes the vasculature, lung and kidney. Tenascin-C is a matrix protein with a restricted expression pattern; it is specifically up-regulated at sites of fibronectin fibril assembly during development and in remodeling adult tissues. Here we demonstrate that specific domains of tenascin-C inhibit fibronectin matrix assembly whereas full-length tenascin-C does not. These domains act via distinct mechanisms: TNfn1-8 blocks fibrillogenesis by binding to fibronectin fibrils and preventing intermolecular fibronectin interactions whilst FBG acts independently of binding to fibronectin and instead is internalized and causes cytoskeletal re-organization. We also show that TNfn1-8 disrupts epithelial cell tubulogenesis. Our data demonstrate that tenascin-C contains cryptic sites which can control tissue levels of fibrillar fibronectin either by preventing de novo fibril assembly or reducing levels of deposited fibronectin. Exposure of these domains during tissue remodeling may provide a novel means of controlling fibronectin assembly and tubulogenic processes dependent on the assembly of this matrix.

Original publication

DOI

10.1016/j.matbio.2010.08.003

Type

Journal article

Journal

Matrix Biol

Publication Date

09/2010

Volume

29

Pages

573 - 585

Keywords

Animals, Binding, Competitive, Cell Line, Cell Shape, Cytoskeleton, Dogs, Endocytosis, Epithelial Cells, Extracellular Matrix, Fibroblasts, Fibronectins, Focal Adhesions, Gene Expression, Heparin, Hepatocyte Growth Factor, Humans, Mice, NIH 3T3 Cells, Peptide Fragments, Protease Inhibitors, Protein Binding, Protein Interaction Domains and Motifs, Protein Structure, Tertiary, Recombinant Proteins, Tenascin