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Zymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly on proteolytic activity. This unit presents a representative zymography protocol for the study of matrix metallopeptidases (MMPs).

Type

Chapter

Publication Date

11/2004

Volume

Chapter 21

Pages

Unit - 21.15

Addresses

Imperial College London, London, United Kingdom.

Keywords

Matrix Metalloproteinases, Electrophoresis, Polyacrylamide Gel, Substrate Specificity, Hydrolysis, Molecular Weight