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Three strategies have been designed to concentrate infectious retroviral vectors from the supernatants of human- (HT1080) and murine- (NIH 3T3) based packaging cells. Streptavidin-conjugated paramagnetic particles in conjunction with (i) antibodies directed against murine fibronectin, (ii) biotinylated lectins, or (iii) biotin-modified packaging cell-surface proteins allow affinity-mediated magnetic concentration of retroviral vectors. Retroviral titers (assayed by colony formation of human myeloid K562 cells) are increased by 1-4 x 10(3)-fold after volume reductions of only 125-fold. Using these procedures, preparations of 5 x 10(8) cfu/ml are routinely made from relatively low-titer (2-5 x 10(5) cfu/ml) starting material. High-titer (paramagnetic) retroviral vector preparations can be used for magnetic field-dependent retroviral infection in vitro. Magnetic field-dependent localization such as this may enable the in vivo administration of formulations that concentrate retroviral infection to the required target tissues and organs.

Original publication




Journal article


Mol ther

Publication Date





623 - 630


3T3 Cells, Animals, Biotin, Cell Line, Cross-Linking Reagents, DNA-Binding Proteins, Fibronectins, Gene Transfer Techniques, Genetic Vectors, HeLa Cells, Humans, K562 Cells, Lectins, Magnetics, Mice, Microscopy, Fluorescence, Plant Proteins, Protein Synthesis Inhibitors, Puromycin, Retroviridae, Streptavidin, Succinimides