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Prior studies demonstrated that NK cells isolated from adult peripheral blood kill the HLA-A-, HLA-B-, and HLA-C-deficient B lymphoblastoid cell line 721.221, but many are unable to kill 721.221 cells transfected with HLA-G, a molecule expressed preferentially on fetal cytotrophoblasts. To determine the biologic relevance of this recognition, we established NK cell clones from the placenta and demonstrate that these NK cells also were unable to kill 721.221 cells expressing HLA-G. Recognition of HLA-G by NK cells was prevented in the presence of anti-CD94 mAb, implicating CD94/NKG2 as the predominant inhibitory NK cell receptor for HLA-G used by decidual NK cells. In contrast, mAbs against the killer cell inhibitory receptors recognizing HLA-Cw3-related, HLA-Cw4-related, or HLA-Bw4 ligands did not affect NK cell killing of the HLA-G transfectants.

Type

Journal article

Journal

Journal of immunology (Baltimore, Md. : 1950)

Publication Date

08/1997

Volume

159

Pages

1072 - 1075

Addresses

Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.

Keywords

Decidua, Killer Cells, Natural, Cell Line, Humans, Membrane Glycoproteins, Lectins, C-Type, Receptors, Immunologic, Receptors, Mitogen, Antigens, CD, Histocompatibility Antigens Class I, HLA Antigens, Cytotoxicity, Immunologic, Adult, Female, NK Cell Lectin-Like Receptor Subfamily D, Receptors, Natural Killer Cell, NK Cell Lectin-Like Receptor Subfamily C