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The acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level of mRNA as IL-1 beta mRNA is more abundant than IL-1 alpha mRNA. Studies of transcription in isolated nuclei however indicate that each gene is transcribed at a similar rate, suggesting that post-transcriptional mechanisms regulate the relative abundance of IL-1 alpha and IL-1 beta mRNA. Measurement of RNA half life after addition of alpha-amanitin (an inhibitor of RNA polymerase II) indicate that IL-1 alpha mRNA is not as stable as IL-1 beta mRNA suggesting one mechanism for the different relative levels of RNA.

Original publication

DOI

10.1016/s0006-291x(88)80919-7

Type

Journal article

Journal

Biochemical and biophysical research communications

Publication Date

10/1988

Volume

156

Pages

830 - 839

Addresses

Charing Cross Sunley Research Centre, London, United Kingdom.

Keywords

Tumor Cells, Cultured, Humans, Leukemia, Monocytic, Acute, Tetradecanoylphorbol Acetate, Cycloheximide, Amanitins, RNA Polymerase II, Lipopolysaccharides, RNA, Messenger, Interleukin-1, Transcription, Genetic, Gene Expression Regulation, Kinetics, Half-Life, Female