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We have established previously that human thyroid epithelial cells (TEC) from patients with autoimmune thyroiditis are able to synthesize cytokines, such as interleukin-1 (IL-1) and interleukin-6 (IL-6). This paper examines TEC in sections from autoimmune thyroiditis for the in vivo production of tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) using the combined techniques of in situ hybridization and immunohistochemistry. Thyroid tissue from patients with Graves' disease, Hashimoto's disease and non-toxic goitre was examined and both mRNA and the protein of TNF-alpha were detected in TEC on frozen sections. Representative figures of only Graves' samples are illustrated in this paper. In contrast, using the same methods, IFN-gamma was detected only in the infiltrating cells and not in TEC of thyroid tissue from the patients.

Type

Journal article

Journal

Immunology

Publication Date

03/1992

Volume

75

Pages

456 - 462

Addresses

Kennedy Institute of Rheumatology, London, U.K.

Keywords

Thyroid Gland, Epithelium, Humans, Goiter, Thyroiditis, Thyroiditis, Autoimmune, Tumor Necrosis Factor-alpha, RNA, Messenger, Nucleic Acid Hybridization, Graves Disease, Interferon-gamma