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The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4(+) T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.

Original publication

DOI

10.1038/ni1210

Type

Journal article

Journal

Nature immunology

Publication Date

07/2005

Volume

6

Pages

707 - 714

Addresses

Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA.

Keywords

Lymph Nodes, Dendritic Cells, CD4-Positive T-Lymphocytes, Animals, Mice, Inbred C57BL, Mice, Transgenic, Mice, Ovalbumin, Bacterial Proteins, Lectins, C-Type, Luminescent Proteins, Receptors, Cell Surface, Antigens, CD, Minor Histocompatibility Antigens, Microscopy, Confocal, Lymphocyte Activation, Specific Pathogen-Free Organisms, Cell Communication, Cell Movement, Immune Tolerance