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The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4(+) T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.

Original publication




Journal article


Nat immunol

Publication Date





707 - 714


Animals, Antigens, CD, Bacterial Proteins, CD4-Positive T-Lymphocytes, Cell Communication, Cell Movement, Dendritic Cells, Immune Tolerance, Lectins, C-Type, Luminescent Proteins, Lymph Nodes, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Confocal, Minor Histocompatibility Antigens, Ovalbumin, Receptors, Cell Surface, Specific Pathogen-Free Organisms