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The Tec family of protein tyrosine kinases plays an important role in T cell signaling. Tec, the prototypical member of this kinase family, can interact with CD28, which is a costimulatory molecule. However, the regulation of Tec upon CD28 stimulation remains poorly understood. Here we show that CD28-B7-mediated interactions are likely involved in the relocalization of Tec at the contact zone between T cells and APC. Upon CD28 ligation with specific antibodies or natural ligands, Tec translocates to the plasma membrane where it colocalizes with the CD28 molecule. The Src-homology 3(SH3) domain of Tec and the two proline-rich motifs of CD28 are involved in this process. Furthermore, we show that CD28 signaling requires the SH3 domain of Tec as well as proline residues present in the intracytoplasmic tail of CD28. These results should provide new insights into the complex regulation of Tec kinases in T cells.

Original publication

DOI

10.1002/eji.200324777

Type

Journal article

Journal

European journal of immunology

Publication Date

07/2004

Volume

34

Pages

1972 - 1980

Addresses

INSERM UMR 599, Equipe labellisée 2001 par la Ligue Nationale contre le Cancer, Université de la Méditerranée, Marseille, France.

Keywords

Antigen-Presenting Cells, T-Lymphocytes, Cell Line, Tumor, L Cells (Cell Line), Cell Membrane, Animals, Humans, Mice, Proline, Antigens, CD28, Antigens, CD80, Interleukin-2, Signal Transduction, Amino Acid Motifs, src Homology Domains, Protein Binding, Protein-Tyrosine Kinases