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Recent studies on genome-wide localization of transcription factor (TF) binding to DNA have shown that a large proportion of identified sequences do not contain consensus motifs predicted by databases of transcription factor binding sites, such as TRANSFAC. The main limitation of these databases is that they are based on a literature search of published examples of binding; consequently the data are not from a systematic survey and may be subject to sampling biases if investigators focused on particular motifs. Thus, there is an urgent need for systematic profiling of vertebrate transcription factor binding to DNA. We have developed a high-throughput platform for the quantitative analysis of protein-DNA interactions based on microarray technology.

Original publication




Journal article


Methods in molecular biology (Clifton, N.J.)

Publication Date





261 - 280


Wellcome Trust Center for Human Genetics, University of Oxford, Oxford, UK.


Humans, DNA-Binding Proteins, Recombinant Proteins, Transcription Factors, DNA, Oligonucleotide Array Sequence Analysis, Cloning, Molecular, Binding Sites, Base Sequence, Protein Binding, Octamer Transcription Factor-1, In Vitro Techniques