Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The extracellular matrix molecule tenascin-C (TNC) has received a lot of attention since its discovery 30 years ago because of its multiple roles in tissue repair, and in pathologies such as chronic inflammation, fibrosis, and cancer. Mouse models with high or no TNC expression have enabled the validation of key roles for TNC in immunity and angiogenesis. In parallel, many approaches including primary cell or organ cultures have shed light on the cellular and molecular mechanisms by which TNC exerts its multiple actions in vivo. Here, we will describe assays that investigate its antiadhesive properties and that measure the effect of TNC on the actin cytoskeleton, cell survival, proliferation, and migration. We will also describe assays to assess the impact of TNC on endothelial and immune cells in cell and organ culture, and to compare the responses of fibroblasts from normal and diseased tissues.

Original publication

DOI

10.1016/bs.mcb.2017.08.023

Type

Journal article

Journal

Methods in cell biology

Publication Date

01/2018

Volume

143

Pages

401 - 428

Addresses

Inserm U1109, The Microenvironmental Niche in Tumorigenesis and Targeted Therapy (MNT3) Team, Strasbourg, France; Université de Strasbourg, LabEx Medalis, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.