A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells
Abu-Shah E., Demetriou P., Balint S., Mayya V., Kutuzov MA., Dushek O., Dustin ML.
<jats:title>Abstract</jats:title><jats:p>Research in the field of human immunology is restricted by the lack of a system that reconstitutes the <jats:italic>in-situ</jats:italic> activation dynamics of quiescent human antigen-specific T-cells interacting with dendritic cells. Here we report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell. Our approach facilitates real-time single cell manipulations, tracking of interactions and functional responses complemented by population-based measurements of cytokines, activation status and proliferation. As a proof of concept, we recapitulate immunological phenomenon such as CD4 help to CD8 T-cells through enhanced maturation of DCs and effect of PD-1 checkpoint blockades. In addition, we characterise unique dynamics of T-cell/DC interactions as a function of antigen affinity.</jats:p>