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Using resting chondrocytes derived from human articular femoral head and a conditionally immortalised human articular chondrocyte cell line we have studied the expression of members of the novel metalloproteinase/disintegrin family termed ADAM. Using RT-PCR we can detect the expression of ADAM-12 a novel family member isolated from myeloma cells [1]. We also find expression of ADAM 10 a functional metalloproteinase/disintegrin first isolated from bovine brain and ADAM-15 a metallodisintegrin isolated from mammary derived epithelial cells. Northern blotting was used to confirm expression. One main transcript is visible for ADAM-12 whereas both ADAM-10 and ADAM-15 have multiple transcripts indicating possible RNA variants potentially derived from alternative splicing or alternative use of polyadenylation sites. Since chondrocytes are proposed as an important source of metalloproteinase enzymes involved in joint pathology the potential relevance of the expression of these molecules to connective tissue disorders is discussed.

Original publication

DOI

10.1006/bbrc.1996.5957

Type

Journal article

Journal

Biochem biophys res commun

Publication Date

13/01/1997

Volume

230

Pages

335 - 339

Keywords

ADAM Proteins, ADAM10 Protein, ADAM12 Protein, Aged, Aged, 80 and over, Alternative Splicing, Amino Acid Sequence, Amyloid Precursor Protein Secretases, Animals, Base Sequence, Brain, Cartilage, Articular, Cattle, Cell Line, Transformed, Cell Membrane, Cells, Cultured, DNA Primers, Female, Humans, Membrane Proteins, Metalloendopeptidases, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Amino Acid, Transcription, Genetic