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Bovine articular chondrocytes were maintained in high density pellet cultures with and without serum and nucleotide triphosphates for different periods of time. Despite half-lives in culture of about 3 h, adenosine triphosphate and uridine triphosphate in the presence of serum increased sulphated glycosaminoglycan and collagen deposition above control levels. In the presence of serum a single dose of uridine triphosphate on the first day of culture was sufficient to induce significant increases in subsequent proteoglycan and collagen deposition. We conclude that both adenine triphosphate and uridine triphosphate are anabolic for articular chondrocytes, and that this effect on the chondrocyte is long-term.

Original publication

DOI

10.1016/s0925-4439(00)00055-7

Type

Journal article

Journal

Biochim biophys acta

Publication Date

18/10/2000

Volume

1502

Pages

297 - 306

Keywords

Adenosine Triphosphate, Animals, Cartilage, Articular, Cattle, Cell Count, Cell Survival, Cells, Cultured, Chondrocytes, Collagen, Culture Media, Serum-Free, Half-Life, Histocytochemistry, Minerals, Proteoglycans, Uridine Triphosphate