Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We have studied how stimulation of protein kinase C and cAMP-dependent protein kinases affect the development of mesencephalic dopaminergic neurons in vitro. IGF-I and bFGF did not activate either second messenger system nor affect the survival of dopaminergic neurons but stimulated dopamine uptake per neuron. Phorbol esters, which stimulate protein kinase C, had no effect on dopamine uptake. Dibutyryl-cAMP caused an increase in dopamine uptake, which was blocked with (Rp)-cAMPS, a specific inhibitor of cAMP-dependent protein kinases. Treating cells with specific phosphodiesterase type IV inhibitors elevated the forskolin-induced increase in dopamine uptake. Furthermore, cAMP, but neither bFGF nor activation dependent astrocyte factor (ADAF), was able to prevent the degeneration of dopaminergic neurons induced by MPP+. These results suggest that increased intracellular cAMP protects dopaminergic neurons in situations of stress and therefore reveal novel possibilities for the treatment of Parkinson's disease.

Type

Journal article

Journal

Journal of neural transmission. Supplementum

Publication Date

01/1995

Volume

46

Pages

217 - 228

Addresses

Preclinical Research, Sandoz Pharma Ltd, Basel, Switzerland.

Keywords

Neurons, Cells, Cultured, Animals, Rats, Rats, Sprague-Dawley, Dopamine, Phorbol Esters, 1-Methyl-4-phenylpyridinium, Fibroblast Growth Factor 2, Insulin-Like Growth Factor I, Cyclic AMP, Neuroprotective Agents, Biological Factors, Cell Differentiation, Cell Survival