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The radioactively labelled constituents present in bone matrix were compared 12 days after injection of either [(14)C]glucosamine or plasma [(14)C]glycoprotein. Both precursors are utilized in the synthesis of organic matrix by bone tissue. Cortical bone from animals injected with [(14)C]glucosamine contains radioactivity derived from glucosamine and plasma glycoproteins and all glycoprotein fractions are labelled. Plasma [(14)C]glycoprotein labels the less acidic glycoproteins to a greater extent than the more acidic components. An antibody has been raised against the less-acidic-glycoprotein fraction of bone. The latter contains a glycoprotein of alpha-mobility that appears to be concentrated specifically in bone tissue and which is present also in plasma. This alpha-glycoprotein accounts for a large proportion of the components labelled and retained in bone matrix after [(14)C]glucosamine injection.

Original publication

DOI

10.1042/bj1360125

Type

Journal article

Journal

The Biochemical journal

Publication Date

09/1973

Volume

136

Pages

125 - 134

Keywords

Bone Matrix, Animals, Goats, Sheep, Rabbits, Guinea Pigs, Carbon Radioisotopes, Microbial Collagenase, Glucosamine, Glycoproteins, Albumins, Serum Albumin, Blood Proteins, Immune Sera, Antibodies, Blood Protein Electrophoresis, Immunoelectrophoresis, Chromatography, DEAE-Cellulose, Electrophoresis, Polyacrylamide Gel, Blood Volume