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The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting.

Original publication




Journal article


Methods mol biol

Publication Date





51 - 63


Caspase, Caspase-11, Caspase-4, Epithelial cells, Gasdermin D, Lactate dehydrogenase, Lipopolysaccharides, Macrophages, Monocytes, Non-canonical inflammasome, Pyroptosis