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BACKGROUND: Erythrocyte deformability is impaired in sickle cell disease (SCD). The regulation of cytoskeletal protein organization plays a key role in erythrocyte deformability. The activation of adenylyl cyclase (AC)/cAMP/Protein kinase A (PKA) signaling pathway was associated with increased deformability in healthy erythrocytes, however the role of this pathway in SCD is unknown. OBJECTIVE: We evaluated mechanical responses of sickle red blood cells under physiological levels of shear stress and the possible link between their deformability and AC/cAMP/PKA signaling pathway. METHODS: The shearing of sickle red blood cells at physiological level (5 Pa) and the measurement of deformability were performed by a laser assisted optical rotational cell analyzer (LORRCA). RESULTS: Red blood cell deformability increased of 2.5-6.5% by blocking the activity of phosphodiesterase with Pentoxifylline (10μM) (p 

Original publication




Journal article


Clin hemorheol microcirc

Publication Date





531 - 543


Protein kinase a, adenylyl cyclase, phosphodiesterase, red blood cell deformability, sickle cell disease, Adenylyl Cyclases, Adult, Anemia, Sickle Cell, Cyclic AMP-Dependent Protein Kinases, Erythrocytes, Erythrocytes, Abnormal, Female, Humans, Male, Young Adult