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Imaging of the immunological synapse (IS) between dendritic cells (DCs) and T cells in suspension is hampered by suboptimal alignment of cell-cell contacts along the vertical imaging plane. This requires optical sectioning that often results in unsatisfactory resolution in time and space. Here, we present a workflow where DCs and T cells are confined between a layer of glass and polydimethylsiloxane (PDMS) that orients the cells along one, horizontal imaging plane, allowing for fast en-face-imaging of the DC-T cell IS.

Original publication




Journal article


Methods mol biol

Publication Date





137 - 147


Dendritic cell, Imaging, Immunological synapse, PDMS, T cell, T-Lymphocytes, Immunological Synapses, Dendritic Cells, Lymphocyte Activation