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A number of matrix metalloproteinases (MMPs) and their endogenous inhibitors called tissue inhibitors of metalloproteinases (TIMPs) from natural sources have been identified. This chapter describes the purification methods of MMPs-1, -2, -3, -7, -8, -9, -10, -12, and -13 and TIMPs-1 and -2. The sources of the proteins and assay methods to detect their activities are also described.

Original publication

DOI

10.1007/978-1-60327-299-5_8

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2010

Volume

622

Pages

123 - 155

Addresses

Department of Physical Pharmacy, Meiji Pharmaceutical University, Tokyo, Japan.

Keywords

Animals, Humans, Rats, Enzyme Precursors, Metalloproteases, Tissue Inhibitor of Metalloproteinases, Molecular Biology, Enzyme Activation