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Five distinct membrane-type matrix metalloproteinases (MT-MMP) have been reported by cDNA cloning. However, the mt4-mmp gene product (MMP-17) has not been identified yet in spite of the cDNA isolation [Puente et al. (1996), Cancer Res. 56, 944-949]. In this study, we re-examined the transcripts for human mt4-mmp by 5' RACE and identified two types of transcripts. The minor one corresponded to the cDNA reported by Puente et al. and failed to express protein, and the other is the major transcript that has an extended open reading frame and expressed 67 and 71 kDa translation products. Thus, functional mt4-mmp has been identified for the first time.

Original publication




Journal article


FEBS letters

Publication Date





353 - 356


Department of Cancer Cell Research, Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, Japan.


COS Cells, Tumor Cells, Cultured, Animals, Humans, Mice, Carcinoma, Breast Neoplasms, Metalloendopeptidases, Matrix Metalloproteinases, DNA, Complementary, DNA Primers, Blotting, Western, Nucleic Acid Amplification Techniques, Reverse Transcriptase Polymerase Chain Reaction, Protein Biosynthesis, Transcription, Genetic, Amino Acid Sequence, Molecular Sequence Data, Matrix Metalloproteinases, Membrane-Associated