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Three monoclonal antibodies which recognize rabbit leucocytes have been characterized by immunofluorescence staining of a variety of cell populations and also by immunochemical techniques. The evidence obtained suggests that these antibodies recognize the rabbit equivalents of the CD58/LFA-3 (VC21), CD43/leukosialin (L11/135) and CD9 (MM2/57) antigens. A fourth antibody, RPN3/57, recognizes an antigen expressed strongly on T cells, thymocytes and neutrophils and at lower levels on platelets. It has not, however been possible to characterize the antigen recognized by RPN3/57 in molecular terms. Both L11/135 and RPN3/57 are useful reagents for the detection of T cells both by flow cytometry and by immunohistochemistry.


Journal article



Publication Date





625 - 630


Animals, Antibodies, Monoclonal, Antigens, Differentiation, Blood Platelets, Erythrocytes, Leukocytes, Mice, Mice, Inbred BALB C, Molecular Weight, Monocytes, Neutrophils, Rabbits, Species Specificity, T-Lymphocytes