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Whole-genome genetic association studies in outbred mouse populations represent a novel approach to identifying the molecular basis of naturally occurring genetic variants, the major source of quantitative variation between inbred strains of mice. Measuring multiple phenotypes in parallel on each mouse would make the approach cost effective, but protocols for phenotyping on a large enough scale have not been developed. In this article we describe the development and deployment of a protocol to collect measures on three models of human disease (anxiety, type II diabetes, and asthma) as well as measures of mouse blood biochemistry, immunology, and hematology. We report that the protocol delivers highly significant differences among the eight inbred strains (A/J, AKR/J, BALBc/J, CBA/J, C3H/HeJ, C57BL/6 J, DBA/2 J, and LP/J), the progenitors of a genetically heterogeneous stock (HS) of mice. We report the successful collection of multiple phenotypes from 2000 outbred HS animals. The phenotypes measured in the protocol form the basis of a large-scale investigation into the genetic basis of complex traits in mice designed to examine interactions between genes and between genes and environment, as well as the main effects of genetic variants on phenotypes.

Original publication

DOI

10.1007/s00335-005-0112-1

Type

Journal article

Journal

Mamm genome

Publication Date

02/2006

Volume

17

Pages

129 - 146

Keywords

Animals, Anxiety, Asthma, Behavior, Animal, Blood Glucose, Data Interpretation, Statistical, Diabetes Mellitus, Type 2, Female, Genome, Glucose Tolerance Test, Immunophenotyping, Insulin, Male, Mice, Mice, Inbred Strains, Phenotype, Plethysmography, Quantitative Trait, Heritable, Species Specificity, Wound Healing