Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

In the present study we have characterized the gamma/delta T cell receptor (TcR) population in synovial fluid (SF) and peripheral blood (PB) of patients with chronic inflammatory arthritis. By double staining we have shown that (a) synovial V delta 1+ cells have a high expression of activation markers CD45R0 ("memory cells") and HLA-DR as compared to PB, indicating a preactivated population of V delta 1-carrying T cells in vivo and (b) interleukin 2-induced expansion of synovial cells yields a high proportion of gamma/delta in most samples expressing predominantly the V delta 1 TcR. Junctional sequence analysis of the TcR delta chain from interleukin 2-expanded PB cell lines demonstrated a polyclonal V delta 1 population in three out of three samples. In SF cell lines three out of four samples were polyclonally expanded. In SF from one patient, however, a limited repertoire of expressed V delta 1 genes was found. Altogether, our data demonstrate the presence of preactivated V delta 1-expressing cells in the synovial compartment. This V delta 1 population is predominantly polyclonal, except in one patient where oligoclonally expanded V delta 1 cells were detected.

Original publication

DOI

10.1002/eji.1830220240

Type

Journal article

Journal

Eur j immunol

Publication Date

02/1992

Volume

22

Pages

567 - 574

Keywords

Antigens, CD, Arthritis, Rheumatoid, Base Sequence, Flow Cytometry, Gene Rearrangement, delta-Chain T-Cell Antigen Receptor, HLA-DR Antigens, Histocompatibility Antigens, Humans, Integrin beta1, Leukocyte Common Antigens, Lymphocyte Activation, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction, RNA, Messenger, Receptors, Antigen, T-Cell, gamma-delta, Synovial Fluid, T-Lymphocyte Subsets