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The induction of antigen-specific suppressor cells in vitro, using high concentrations (100 mug/ml) of keyhole limpet hemocyanin (KLH) in Marbrook flasks is described. Spleen and cortisone-resistant thymocytes were the richest source of suppressor cell precursors, compared to lymph node cells, peripheral blood lymphocytes or thoracic duct lymphocytes. Suppressor cells induced with KLH only suppressed KLH-reactive helper cells, and not B cells or helper cells of other specificity. The suppressor cells were T cells, as judged by their sensitivity to anti-Thy-1.2, heterologous anti-T, but not anti-B antisera.

Type

Journal article

Journal

European journal of immunology

Publication Date

04/1976

Volume

6

Pages

296 - 301

Keywords

Lymph Nodes, Spleen, T-Lymphocytes, Cells, Cultured, Animals, Mice, Inbred CBA, Mice, Cortisone, Hemocyanin, Antigens, Epitopes, Immunosuppression, Antibody Formation, Drug Resistance, Kinetics