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This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real-time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes.

Original publication

DOI

10.1002/0471142735.im1428s96

Type

Journal article

Journal

Current protocols in immunology

Publication Date

02/2012

Volume

Chapter 14

Addresses

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.

Keywords

Macrophages, Humans, RNA, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Gene Expression Regulation, Gene Silencing, Real-Time Polymerase Chain Reaction