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This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real-time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes.

Original publication




Journal article


Current protocols in immunology

Publication Date



Chapter 14


Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.


Macrophages, Humans, RNA, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Gene Expression Regulation, Gene Silencing, Real-Time Polymerase Chain Reaction