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Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.

Original publication




Journal article


Methods mol biol

Publication Date





85 - 93


CD4+ T cells, CD45RA+, Human T cell, Lentiviral vector, Lentivirus, Primary, Titer, Gene Transfer Techniques, Genetic Vectors, HIV-1, Humans, Lentivirus, Primary Cell Culture, T-Lymphocytes, Transduction, Genetic, Transfection, Transgenes