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Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated.


Journal article


Arch oral biol

Publication Date





337 - 345


Animals, Bone and Bones, Chondroitin Sulfates, Chromatography, Edetic Acid, Electrophoresis, Polyacrylamide Gel, Glycosaminoglycans, Keratan Sulfate, Macromolecular Substances, Proteoglycans, Rabbits, Sialoglycoproteins