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An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in vivo and that they are "right-side-out". The disruption of core protein-fibres appears to be a prerequisite for vesicle formation.

Type

Journal article

Journal

Journal of cell science

Publication Date

02/1981

Volume

47

Pages

227 - 236

Keywords

Intestinal Mucosa, Duodenum, Cell Membrane, Microvilli, Animals, Rats, Microscopy, Electron, Male